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Protein Biochemistry
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Protein Extraction Kits
Plasma Membrane Protein Extraction Kit
Plasma Membrane Protein Extraction Kit from Abcam
Be the first to write a review!
Citations:
(88)
Supplier Page
Supplier Page from
Abcam for
Plasma Membrane Protein Extraction Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Plasma Membrane Protein Extraction Kit
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab65400
Quantity
50 test
Time
1h 0m
Target
Plasma Membrane protein extraction
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Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (88)
(1)
Targeting endoplasmic reticulum associated degradation pathway combined with radiotherapy enhances the immunogenicity of esophageal cancer cells
Cancer Biology & Therapy
December 31, 2023
Hui Luo, Yanan Sun, Liuxiang Wang, Hui Liu, Ran Zhao, Mengqiu Song, Hong Ge
were isolated from the total
membrane
component by using the
plasma membrane
protein extraction
kit (
ab65400
,
Abcam
, UK). Cell surface CLAR and sodium-potassium ATPase (Na+/K+ ATPase) were detected in the
western blot
(2)
Receptor-interacting protein 1 and 3 kinase activity are required for high-fat diet induced liver injury in mice
Front Endocrinol (Lausanne)
December 15, 2023
Xiaoqin Wu, Rakesh K. Arya, Emily Huang, Megan R. McMullen, Laura E. Nagy
from murine liver Plasma fractions were isolated using the
plasma membrane
protein extraction
kit (
ab65400
,
Abcam
). Liver tissues were resuspended in homogenization buffer and lysed using a Dounce homogenizer
(3)
Targeting protein methylation in pancreatic cancer cells results in KRAS signaling imbalance and inhibition of autophagy
Cell Death & Disease
November 23, 2023
María F. Montenegro, Román Martí-Díaz, Ana Navarro, Jorge Tolivia, Luis Sánchez-del-Campo, Juan Cabezas-Herrera, José Neptuno Rodríguez-López
Cell fractionation
The
plasma membrane
fraction was isolated using a
plasma membrane
protein extraction
kit (
Abcam
;
ab65400
) following the manufacturer’s instructions. In brief, cells were lysed in homogenization buffer containing
(4)
Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscle
Nature Communications
August 17, 2023
Anastasia Sacharidou, Ken Chambliss, Jun Peng, Jose Barrera, Keiji Tanigaki, Katherine Luby-Phelps, İpek Özdemir, Sohaib Khan, Shashank R. Sirsi, Sung Hoon Kim,
et al.
were isolated from total cellular
membranes
by phase separation (
Abcam
plasma membrane
extraction kit;
ab65400
). Following treatment, the cells were washed with ice cold PBS and pelleted twice, and then homogenized
(5)
An insulin-regulated arrestin domain protein controls hepatic glucagon action
The Journal of biological chemistry
August 1, 2023
Dagdeviren S, Hoang MF, Sarikhani M, Meier V, Benoit JC, Okawa MC, Melnik VY, Ricci-Blair EM, Foot N, Friedline RH,
et al
.
Plasma Membrane isolation kit from
Abcam
that removes all cellular
membrane
proteins (catalog no.:
ab65400
). For quantitative
RT–PCR
, RNA was extracted from homogenized liver samples by the TRIzol (Life Sciences)
(6)
MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR
Cancer communications (London, England)
July 1, 2023
Luo L, Wei D, Pan Y, Wang QX, Feng JX, Yu B, Kang T, Luo J, Yang J, Gao S.
experiments.
Plasma membrane
proteins were isolated using the
Plasma Membrane Protein Extraction Kit
(
ab65400
,
Abcam
). Briefly, cells were harvested, washed twice with ice‐cold PBS, and resuspended in homogenization
(7)
ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma
Cancer research
June 15, 2023
Sun Y, Zhu L, Liu P, Zhang H, Guo F, Jin X.
intracellular membrane
(ICM) fractions were isolated with the
Plasma Membrane Protein Extraction Kit
(
Abcam
,
ab65400
). Briefly, cells were incubated with 2 mL of homogenized buffer at 4°C after being washed three times
(8)
Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo
Autophagy
April 1, 2023
Wu SY, Wu HT, Wang YC, Chang CJ, Shan YS, Wu SR, Chiu YC, Hsu CL, Juan HF, Lan KY,
et al
.
independent
mice
.
Plasma membrane
protein extraction
A
plasma membrane
protein extraction
kit (
Abcam
,
ab65400
) was used to purify the
cytosol
and
plasma membrane
fraction from MIN6 cells
after treatment
of LG or
(9)
Defective BVES-mediated feedback control of cAMP in muscular dystrophy
Nature communications
March 30, 2023
Li H, Wang P, Zhang C, Zuo Y, Zhou Y, Han R.
(Bio-Rad, Hercules, CA). The
membrane
extraction was performed using the Membrane Protein Extraction Kit (
ab65400
,
Abcam
, Waltham, MA) according to the manufacturer’s instruction. The extracted protein samples were
(10)
Transglutaminase 2 associated with PI3K and PTEN in a membrane-bound signalosome platform blunts cell death
Cell Death & Disease
March 28, 2023
Károly Jambrovics, Pál Botó, Attila Pap, Zsolt Sarang, Zsuzsanna Kolostyák, Zsolt Czimmerer, Istvan Szatmari, László Fésüs, Iván P. Uray, Zoltán Balajthy
[12].
Plasma membrane
preparation The
plasma membrane
was isolated according to the
Abcam
protocol (
ab65400
). PIP3 extraction and measurement The isolation of PIP molecules was carried out by chloroform-methanol
(11)
Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2
Nature communications
February 16, 2023
Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams LA, Stolz A, Lichtenthaler SF, Elazar Z, Behrends C.
subsequently processed as described above (proximity
labeling
). PM enrichment An PM extraction kit (
Abcam
,
Ab65400
) was used according to the manufacturer’s guidelines. Cells were collected by scaping, homogenized in
(12)
Inhibition of sphingosine-1-phosphate receptor 3 suppresses ATP-induced NLRP3 inflammasome activation in macrophages via TWIK2-mediated potassium efflux
Frontiers in immunology
January 1, 2023
Wang Y, Wang C, He Q, Chen G, Yu J, Cang J, Zhong M.
protein separation The
plasma membrane
was isolated using
Plasma Membrane Protein Extraction Kit
(
ab65400
,
Abcam
, UK) according to the manufacturer’s instructions. One 10-cm plate of cells for each condition
(13)
Overexpression of Rhodopsin or Its Mutants Leads to Energy Metabolism Dysfunction in 661w Cells
Investigative Opthalmology & Visual Science
December 5, 2022
Yang Liu, Xin Wang, Ruowen Gong, Gezhi Xu, Min Zhu
and
plasma membrane
proteins were extracted using an
Abcam
Plasma Membrane Protein Extraction Kit
(
ab65400
). Cells were harvested, homogenized, and centrifuged at 700g for 10 minutes. The supernatants were centrifuged
(14)
Geranylgeranyl pyrophosphate depletion by statins compromises skeletal muscle insulin sensitivity
Journal of Cachexia, Sarcopenia and Muscle
December 1, 2022
Lai Wang, Zuguo Zheng, Lijun Zhu, Lingchang Meng, Hanling Liu, Keke Wang, Jun Chen, Ping Li, Hua Yang
information. Isolation of
plasma membrane
fractionation The
Plasma Membrane Protein Extraction Kit
(
ab65400
,
Abcam
) was used to isolate
plasma membrane
proteins from C2C12
myotubes
and primary
mouse
myotubes
(15)
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma
Nature metabolism
October 1, 2022
Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ,
et al
.
from
Sigma
. PalmB (178581) was purchased from Millipore. The
Plasma Membrane Protein Extraction Kit
(
ab65400
) was purchased from
Abcam
.
GoTaq qPCR Master Mix
(
A6001
) was purchased from
Promega
. Sirius red (G1018)
(16)
An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory
Nature cell biology
October 1, 2022
Lanna A, Vaz B, D'Ambra C, Valvo S, Vuotto C, Chiurchiù V, Devine O, Sanchez M, Borsellino G, Akbar AN,
et al
.
telomere
vesicles,
T cell
plasma membranes
were then purified with
Plasma Membrane Protein Extraction Kit
(
ab65400
;
Abcam
) and absence APC
telomeres
assessed by
confocal
imaging on Leica SP2.
Plasma membrane
purification
(17)
Ppt1-deficiency dysregulates lysosomal Ca
++
homeostasis contributing to pathogenesis in a mouse model of CLN1 disease
Journal of inherited metabolic disease
May 1, 2022
Mondal A, Appu AP, Sadhukhan T, Bagh MB, Previde RM, Sadhukhan S, Stojilkovic S, Liu A, Mukherjee AB.
isolation The
plasma membrane
fractions were isolated using
Plasma Membrane Protein Extraction Kit
(
Abcam
, Cat#
ab65400
) according to the manufacturer’s protocol.
Western blot analysis
Western blot
analyses were performed
(18)
Pulmonary endothelial NEDD9 and the prothrombotic pathophenotype of acute respiratory distress syndrome due to SARS-CoV-2 infection
Pulmonary circulation
April 1, 2022
Alba GA, Samokhin AO, Wang RS, Wertheim BM, Haley KJ, Padera RF, Vargas SO, Rosas IO, Hariri LP, Shih A,
et al
.
isolated
plasma membrane
fractions of HPAEC lysates using a
Plasma Membrane Protein Extraction Kit
(
Abcam
,
ab65400
) according to the manufacturer's instructions. Magnetic beads (
Bio‐Rad
Protein G
SureBeads
) were resuspended
(19)
AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration
Communications biology
December 9, 2021
Miyagishima KJ, Sharma R, Nimmagadda M, Clore-Gronenborn K, Qureshy Z, Ortolan D, Bose D, Farnoodian M, Zhang C, Fausey A,
et al
.
Plasma membrane
preparation of iRPE were prepared using a
Plasma Membrane Protein Extraction Kit
(
Abcam
, #
ab65400
) following the manufacturer’s recommendations. In brief, 9 million iRPE cells were collected from transwells
(20)
High glucose induces trafficking of prorenin receptor and stimulates profibrotic factors in the collecting duct
Scientific Reports
December 1, 2021
Venkateswara R. Gogulamudi, Danielle Y. Arita, Camille R. T. Bourgeois, Justine Jorgensen, Jing He, William C. Wimley, Ryosuke Satou, Alexis A. Gonzalez, Minolfa C. Prieto
supplement serum free media for 1, 6 h specified in each protocol. Abcam Plasma Membrane Isolation Kit (
Ab65400
;
ABCAM
, Cambridge) was used to extract the
membrane
proteins.
Western blotting
was performed using antibody
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Plasma Membrane Protein Extraction Kit from Abcam
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